Western blot was performed using Anti-c-Kit Recombinant Rabbit Monoclonal Antibody (17)(Product # MA5-29722) and a 109kDa band corresponding to Mast/stem cell growth factor receptor Kit was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), K-562 (Lane 2), SH-SY5Y (Lane 3), HEL 92.1.7 (Lane 4), SK-BR-3 (Lane 5), Mouse Kidney (Lane 6), Mouse Mammary gland (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Expression of c-KIT was found to be higher in IMR-32, K-562, SH-SY5Y, HEL 92.1.7 and Mouse kidney as compared to SK-BR-3 and Mouse Mammary gland.